LOCUS       40924_23474       11087 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB619 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Nakamura S, Mano S, Tanaka Y, Ohnishi M, Nakamori C, Araki M, Niwa 
            T, Nishimura M, Kaminaka H, Nakagawa T, Sato Y, Ishiguro S.
  TITLE     Gateway binary vectors with the bialaphos resistance gene, bar, as a
            selection marker for plant transformation
  JOURNAL   Biosci. Biotechnol. Biochem. 74 (6), 1315-1319 (2010)
  PUBMED    20530878
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Tanaka Y, Nakamura S, Ishiguro S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11087)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11087)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2010"; volume: "74"; issue: "6"; 
            pages: "1315-1319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2010) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        484..514
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             568..1224
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1569..1871
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1915..2039)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2065..2094
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2101..2130
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2146..2175
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2182..2211
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2218..2247
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2263..2292
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2299..2328
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2335..2364
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2380..2409
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2416..2445
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2473..2725
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2759..2775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2783..2799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2807..2837)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2852..2873)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2909..3161)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3324..3872)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(3924..4103)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4609..4633)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5154..5942
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6191..6779
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6965..7105)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7449..7643)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7712..8782)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9214..9840)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagcg ggttaattaa cggtgaacaa aagctaatct ccgaggaaga cttgaacggt
     2101 gaacaaaaat taatctcaga agaagacttg aacggactcg acggtgaaca aaagttgatt
     2161 tctgaagaag atttgaacgg tgaacaaaag ctaatctccg aggaagactt gaacggtgaa
     2221 caaaaattaa tctcagaaga agacttgaac ggactcgacg gtgaacaaaa gttgatttct
     2281 gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa cggtgaacaa
     2341 aaattaatct cagaagaaga cttgaacgga ctcgacggtg aacaaaagtt gatttctgaa
     2401 gaagatttga acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgcttagagc
     2461 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     2521 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     2581 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     2641 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     2701 gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt
     2761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa
     2881 ttaggcgcgc ctatttctat taattcccga tctagtaaca tagatgacac cgcgcgcgat
     2941 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     3001 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     3061 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     3121 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgagctgggg
     3181 acgtccccag ctcggtaccc cctcctgggc acggtcgtac gcgatccgcc gcggttccac
     3241 ctcaccattg cgggtatgcc agttgagttc gatgagttcc cgtaccagct ccaactcggg
     3301 cgatgccacg cgcactccgt tcagatctcg gtgacgggca ggaccggacg gggcggtacc
     3361 ggcaggctga agtccagctg ccagaaaccc acgtcatgcc agttcccgtg cttgaagccg
     3421 gccgcccgca gcatgccgcg gggggcatat ccgagcgcct cgtgcatgcg cacgctcggg
     3481 tcgttgggca gcccgatgac agcgaccacg ctcttgaagc cctgtgcctc cagggacttc
     3541 agcaggtggg tgtagagcgt ggagcccagt cccgtccgct ggtggcgggg ggagacgtac
     3601 acggtcgact cggccgtcca gtcgtaggcg ttgcgtgcct tccaggggcc cgcgtaggcg
     3661 atgccggcga cctcgccgtc cacctcggcg acgagccagg gatagcgctc ccgcagacgg
     3721 acgaggtcgt ccgtccactc ctgcggttcc tgcggctcgg tacggaagtt gaccgtgctt
     3781 gtctcgatgt agtggttgac gatggtgcag accgccggca tgtccgcctc ggtggcacgg
     3841 cggatgtcgg ccgggcgtcg ttctgggctc atatcttatt gccccccggg atcagatctg
     3901 gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
     3961 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
     4021 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag
     4081 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
     4141 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcttga tcccctgcgc
     4201 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     4261 ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct
     4321 agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc
     4381 cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg
     4441 gctttctacg tgttccgctt cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt
     4501 gaagctgatc cgtcgagatt aatagaaata aattcagcct aattcggcgt taattcagta
     4561 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     4621 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     4681 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     4741 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     4801 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     4861 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     4921 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     4981 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     5041 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     5101 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     5161 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     5221 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     5281 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     5341 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     5401 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     5461 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     5521 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     5581 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     5641 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     5701 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     5761 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     5821 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     5881 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     5941 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     6001 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     6061 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     6121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6301 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     6361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6901 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6961 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     7021 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     7081 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     7141 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7201 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     7261 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     7321 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     7381 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     7441 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     7501 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     7561 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     7621 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     7681 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     7741 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     7801 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     7861 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     7921 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     7981 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     8041 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     8101 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     8161 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     8221 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     8281 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     8341 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     8401 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     8461 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     8521 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     8581 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     8641 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
     8701 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
     8761 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
     8821 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
     8881 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
     8941 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
     9001 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
     9061 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
     9121 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
     9181 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
     9241 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
     9301 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
     9361 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
     9421 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
     9481 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
     9541 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
     9601 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
     9661 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
     9721 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
     9781 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
     9841 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
     9901 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
     9961 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    10021 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    10081 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    10141 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    10201 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    10261 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    10321 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    10381 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    10441 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    10501 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    10561 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    10621 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    10681 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    10741 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    10801 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    10861 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    10921 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    10981 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
    11041 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
